Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. In this technique, molecules are separated according to size and electrical charge. It is of utmost importance as the samples that need to be analyzed are then loaded into tiny wells on the gel with the help of a pipette.
Once the load is ready, an electric current of 50-150 V is applied. Now, the charged molecules present in the sample begin to migrate through the gel towards the electrodes.
The speed at which each molecule travels through the gel is called its electrophoretic mobility and is primarily determined by its net charge and size. Strongly charged molecules move faster than weakly charged ones.
Once the separation is complete, the gel is stained with a dye to reveal the separation bands. Ethidium bromide is a fluorescent dye commonly used in gel electrophoresis. The gel is soaked in dilute ethidium bromide solution and then placed on an ultraviolet transilluminator to visualize the separation bands. The bands are immediately examined or photographed for future reference, as they will diffuse into the gel over time.
Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. In this technique, molecules are separated according to size and electrical charge.
The gel used in gel electrophoresis is generally made from a material called agarose, which is a gelatinous substance extracted from seaweed. This porous gel could be used to separate macromolecules of many different sizes. The gel is immersed in a buffer solution of the salt in an electrophoresis chamber. Tris-borate-EDTA (TBE) is commonly used as the buffer. Its main function is to control the pH of the system. The chamber has two electrodes - one positive and one negative - at its two ends.
Vertical gel electrophoresis is a more complex setup compared to horizontal gel system. Researchers often use this system to separate proteins instead of nucleic acids. However, before separating the proteins, it is necessary to break the quaternary structure of the proteins at the linear strand.
With two gel casts, you can cast 1 to 4 plates of gels at the same time. There are two methods of "casting gel in original position" and "multi casting gel" as an option.
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Horizontal gel electrophoresis uses basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous
Vertical gel electrophoresis is a more complex setup compared to horizontal gel system. Researchers typically use this system to separate proteins rather than nucleic acids. However, before separating the proteins, you must break the structure ...
At Kalstein we have an excellent range of laboratory electrophoresis systems. That is why we invite you to see our product section in the menu.
Agarose gel electrophoresis
Agarose gel is a good electrophoretic support. In addition, its nature gives it the characteristic of being able to separate molecules based on their size. This support allows nucleic acids to separate
Polyacrylamide Gel Electrophoresis
Polyacrylamide gel is considered the best support. They also have great mechanical stability and are insoluble in water. Among so many advantages there is a drawback that limits its use: it is neurotoxic.
Capillary electrophoresis
This technique is very different from the previous ones. The support is different in that it uses fused silica capillaries, covered with a layer of polyimine. Results are viewed through a computer screen.
There are countless models, so it is normal that you do not know which laboratory electrophoresis systems to buy that suits your needs. At Kalstein, we test them to find what you're looking for.
In a generic way, the clinical syndromes that result from alterations in hemoglobin (Hb) are known as hemoglobinopathies and various laboratory tests are interpreted to determine them, of which the most relevant is electrophoresis (EF) of Hb.
Multiple Myeloma; is a multifocal plasma cell neoplasm that affects the bone marrow and is associated with the production of a serum and urinary monoclonal protein. The cause is a progressive unregulated proliferation of plasma cells that accumulate in the bone marrow.
Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA and other proteins according to their respective molecular size and charge. The DNA, RNA, or proteins that need to be separated in this method are run through a gel that ...
Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio, using a gelatinous matrix as a base. This technique has a variety of practical uses, such as forensic medicine for the identification of people, the human genome project, protein and genetic mutation research, and clinical diagnostic tests.
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