Electrophoresis is carried out with equipment consisting of a negative charge at one end and a positive charge at the other, called an electrophoresis system. When charged molecules are inserted into this environment, the negatively charged molecules will go to one end and the positively charged molecules to the other end. For example, when analyzing proteins in a gel, in these machines, the whole protein is taken to analyze its size. In this way, the shorter proteins will migrate to the poles more quickly and will be reflected at the bottom of the gel. The longer ones, on the other hand, will remain at the top.
When a mixture of ionized molecules with a net charge are placed in an electric field, they experience a force of attraction towards the pole with the opposite charge. After a certain time has elapsed, the positively charged molecules will move towards the cathode (negative pole) and the positively charged molecules will move towards the anode (positive pole).
Horizontal gel electrophoresis uses the basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous. The agarose gel is used to separate the gel box into two compartments. One end of the gel box contains an anode, while the other end contains a cathode. When a current is applied, the buffer used in this technique allows the creation of a charge gradient. When the charge is applied, the gel tends to heat up.
The buffer also functions as a coolant, which maintains the temperature at optimal levels. Recirculation of the running buffer prevents the formation of a pH gradient. A batch buffer system cannot be used in horizontal gel electrophoresis since the two compartments of the gel system are connected to the running buffer.
The vertical gel electrophoresis technique works according to the primary theory of gel electrophoresis, but is considered to be more complex than the horizontal gel electrophoresis method.
This technique uses a discontinuous buffer. A cathode is located in the upper chamber, and the anode is located in the lower chamber. The electrodes present in each compartment provide the required electric field. A thin layer of gel is poured between the two mounted glass plates. Thus, the upper part of the gel is immersed in the upper chamber, and the lower part of the gel is immersed in the lower chamber. Once the current is applied, a small portion of the buffer moves into the bottom chamber from the top chamber through the gel.
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally...
YR horizontal gel electrophoresis units have been designed by scientists with the laboratory environment ...
YR horizontal gel electrophoresis units have been designed by scientists with the laboratory environment ...
| Model | YR03430 |
| Glass plate size（W×L） | 216×110 mm |
| Gel Size（W×L） | 186×95 mm |
| Gel thickness | 1.0（mm） |
| Gel amount | 1～2（pieces） |
| Sample volume | （1.0mm）25、40、52wells |
| Buffer Volume | ～1800（ml） |
| Dimension（L×W×H） | 280×140×135（mm） |
| Net weight | 3.2（kg） |
Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size to electric charge ratio, using a...
Electrophoresis is a basic method in the field of molecular biology for the analysis (separation, purification, preparation) of nucleic acids and proteins....
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Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such...
Electrophoresis is an essential laboratory technique in biological studies, and its use is spreading to other types of fields ...
Functionally electrophoresis is a technique that consists of proportional migration of molecules through a gel or other ...
In humans, the genetic link of certain diseases between sex-linked hemophilia and color blindness was first observed...
Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA, and other proteins according to their respective molecular size and charge. The DNA, RNA, or proteins that must be separated in this method...
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