A thermal cycler, also known as a PCR machine or PCR thermal recycler, is an apparatus used in molecular biology that allows the temperature cycles necessary for a DNA amplification polymerase chain reaction or for sequence reactions with the Sanger method. . The most common model consists of an electrical resistance block that distributes a homogeneous temperature through a plate for times that can be programmable, normally with temperature ranges from 4 ° C to 96 ° C. Since the reactions incubated in the apparatus are in aqueous solutions, they usually include a lid plate constantly heated to 103 ° C to avoid condensation of water on the lids of the tubes where the reaction occurs, and thus prevent the solutes from concentrating. , which would modify the optimal conditions for the polymerizing enzyme and the thermodynamics of the initiator pairing. There are also other less popular technologies using hot air distribution in suspended tubes, achieving the same goal of efficiently transferring heat to the reaction so that the temperature cycles change.
A thermal cycler is a molecular biology laboratory equipment, used in the amplification of DNA molecules by means of the PCR (Polymerase Chain Reaction) technique, because it is capable of carrying out cycles of temperatures necessary for them to amplify. the DNA strands.
The PCR process is automatic and can be completed in just a few hours. This process is entirely driven by the thermal cycler, which is programmed to change the reaction temperature every few minutes making denaturation and DNA synthesis possible.
The thermal cycler is different if an end-point PCR or a real-time PCR is performed, since the latter must detect fluorescence and in different absorption channels.
The most common thermal cycler model consists of an electrical resistance block that distributes a homogeneous temperature (between 4ºC and 96ºC) through a plate for a programmable time. In some equipment, the electrical resistance has been replaced by Peltier technology (semiconductors), which allows greater homogeneity in temperature.
The thermal cycler is a device that allows the polymerase chain reaction (PCR) efficiently and quickly; by means of the automatic and cyclical realization of the temperature changes that are required for the amplification of a deoxyribonucleic acid (DNA) chain; from a thermostable enzyme.
Thermal cyclers are used for qualitative amplifications or to quantify the amount of amplified DNA. The results of these DNA amplification techniques have a great impact on our society; especially in this period of pandemic where they have become one of the main diagnostic tests, which is why it must be ensured that the thermal cyclers are accurate; accurate and consistent, in order to obtain reliable results.
The polymerase chain reaction, called PCR for short by its acronym in English (polymerase chain reaction), is a technique that allows the exponential amplification of a DNA fragment of interest. That is, from a low number of copies of this fragment, after the PCR reaction, millions of copies are obtained that are already easily detectable in the laboratory. PCR was invented by Kary Mullis in 1986 and earned him the Nobel Prize in 1993.
YR01869 Thermal Cycler
Extended temperature control mode that is closer to the temperature control of the required experiment and is able to effectively avoid the system error caused by the discrepancy of the temperature points between the temperature of the instrument display, the actual temperature of the block and the required temperature for the reagents. To improve the precision of the experiment and ensure the high efficiency, the strict temperature control debugging program ensures that each instrument can meet the needs of different experiments.
12-channel temperature probes detect simultaneously, ensuring sample temperature homogeneity. The airtight technique can effectively eliminate the marginal effect of PCR. The outside temperature probe technique tracing the inside curve test can effectively guarantee the accuracy of the sample temperature.
If we take it from the perspective of a molecular biologist, this is the best way to calibrate any thermal cycler as long as it is the process that mimics the PCR process. The correct process is to put tuvos in block wells, fill the reaction mix, and place the sensors.
From one perspective, the best way to calibrate an implement such as a thermal cycler is by standard and representative measurement from the PCR process. The method allows the calibration of all standard PCRs
During this process, traceability is guaranteed by using reference standards and expression of values that are in charge of calibrating in SI units. However, also due to that uncertainty calculated from the calibration result.
There are countless models, so it is normal that you do not know which laboratory thermal cycler to buy that suits your needs. At Kalstein, we test them to find what you're looking for.
The thermal cycler is a device that allows the polymerase chain reaction (PCR) efficiently and quickly; by means of the automatic and cyclical realization of the temperature changes that are required for amplification
As is well known a thermal cycler; is a device used in Molecular Biology that allows the necessary temperature cycles to be carried out for the amplification of various DNA strands in the PCR technique
The thermal cycler or sequencer is a device that allows the polymerase chain reaction (PCR) efficiently and quickly; by means of the automatic and cyclical realization of the temperature changes that are required to
A thermal cycler is a molecular biology laboratory equipment used in the amplification of DNA molecules using the PCR (Polymerase Chain Reaction) technique, because it is capable of carrying out temperature cycles necessary for them to amplify.
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