The spectrophotometer, built by advanced manufacturing processes, is one of the leading human-developed diagnostic and research instruments. It is used in the laboratory to determine the concentration of a substance in a solution, allowing for quantitative analysis.

How do these teams work?

As we know, use the properties of light and its interaction with other substances to determine their nature. In general, the light from a special characteristic lamp is guided through a device that selects and separates the light from a certain wavelength and passes it through a sample. The intensity of the light exiting the sample is captured and compared with the intensity of the light that affects the sample and from it, the transmission of the sample is calculated, which depends on factors such as the concentration of the substance. .
As a basic principle, light is considered to be a form of electromagnetic energy, which in vacuum has a constant and universal speed [C] of approximately 3 x 108 M.s-1. In any other (transparent) medium through which light passes, its speed will be slightly slower and can be calculated by the equation V0 = C.N-1. Electromagnetic energy has a wide range of wavelengths. And during the passage or interaction with various media, it presents a series of phenomena, among which reflection, refraction, diffraction, absorption, diffusion, polarization and others used in various instruments and devices are distinguished.


The different types of UV visible spectrometers.

Product categories to select


Double beam spectrophotometer

In the dual beam setup, the source passes through the monochromator and is shared in 2 beams: one directed at the "target", that is, the compartment containing the solvent, the other directed towards the sample compartment. The intensity of the light is measured by 2 photodiodes or photomultiplier.
The sampler compartment is the one placed on the front of the device (see photo).
The dual beam spectrophotometer is more expensive to buy, but also more accurate. After running the blank with both tanks filled with solvent, the device measures the absorbance of the sample while cutting off white variations during measurement. These variations may be due, for example, to fluctuations in the intensity of the lamps if the spectrometer has not been illuminated early enough before analysis.
In a monophaiszyge, the blank is performed once and at the beginning of the analysis and is no longer controlled unless the operator decides to redo it.


Single beam spectrophotometer

A spectrophotometer is the highest frequency double beam. However, some cheap or old instruments can just be done. In a single beam instrument, all the light passes through the cell containing the sample. Reference intensity I 0 {\ DisplayStyle i_ {0}} i_ {0} It is measured by replacing the sample with a reference (same tank and even solvent). This device is the first to be used. It is still found in spectrophotometers designed for teaching or for measurements in industry. In a double beam instrument, the light is separated into two beams before reaching the sample. One of the beams is used as a reference and passes through a "blank" (same tank and even solvent as sample), the other passes through the sample. Some double-beam instruments have two detectors (photodiodes or photomultipliers), and the reference and sample beams are measured at the same time. In other instruments equipped with a single detector, both beams pass through an optical separator, blocking one of the packages at a time. The alternative detector between the measurement of the sample beam and that of the blank.


Our best-selling spectrophotometer

Double Beam Spectrophotometer - YR01862

Double optical path, double beam optical system, double detector, with a high-performance imported network, lower stray light, higher stability, reliability, more accurate analysis.
Equipped with a 10 "touch screen, built-in 32g memory, supports Bluetooth connection network.
Dynamic Features, DNA / Protein Testing, Multiple Wavelength Testing and Data Printing
Adopt the suspension design of the optical system, the entire optical path is independently fixed on the 16mm thick aluminum base without deformation.
Vibrations from the outside have no impact on the optical system, thus improving the stability and reliability of the instrument.


Cheapest laboratory Spectrophotometer models on Offers)


Single Beam Visible Spectrophotometer - YR01844

UV-VIS Spectrophotometer - YR01849

Visible Spectrophotometer - YR01846

Visible Spectrophotometer - YR01845

Espectrofot贸metro UV / VIS de doble haz

Spectrophotometer - YR01850

UV / VIS Spectrophotometer - YR01853

UV-VIS Spectrophotometer - YR01855


What are your ideal spectrophotometers?

There are countless models, so it is normal that you do not know which laboratory spectrophotometer to buy to suit your needs. At Kalstein, we test them to find what you're looking for.

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Kalstein testimonials.


The spectrophotometer is one of the main diagnostic and research instruments developed by man. It is used in the laboratory to determine the concentration of a substance in a solution, allowing for quantitative analysis.

Spectrophotometers, in general, are highly specialized and expensive equipment. Its conservation depends to a large extent on the way of installation and use. The surrounding environment and the quality of the electricity services are of the utmost importance for the equipment to be able to provide services according to the specifications for which it was manufactured. Maintenance procedures that may require varying complexity, ranging from careful component cleaning to specialized procedures, should only be performed by properly trained technicians or engineers with technical information developed by manufacturers.

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