Diagnosis of Cancer in Women with the Microplate Reader

November 1, 2022by Kalstein
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A microplate reader is an instrument that is responsible for reading the results of tests previously performed, by means of measurements of absorbance, luminescence and fluorescence. This equipment, because it has a wide variety of modes of activity, is used in medicine and scientific research, to diagnose and detect biological, physical and chemical anomalies that are present in a sample. One way to diagnose the antigen of, for example, breast cancer, is through the use of a microplate reader, by reading the absorbance of the sample, after the application of an ELISA test.

Cancer is a fatal disease that causes cells in the body to multiply uncontrollably, spreading to other parts of the body. In normal circumstances, cells in the human body multiply through the process of cell division, so that new cells form as old cells age or die. However, with cancer, trillions of abnormal cells can form because they divide uncontrollably.

Breast cancer can be diagnosed by a serologic test, the ELISA test, which helps detect antibodies in the blood. In this test an enzyme reaction is generated in the sample, which causes it to change color, if the reaction is positive. To be able to read the absorbance and check for the presence of antigen in the sample, a microplate reader should be used.

Principle of cancer diagnosis in women with the microplate reader

Cancer is a genetic disease that causes changes and uncontrolled genes, such changes can be caused by various reasons, for example, error in the multiplication of cells, damage to DNA due to environmental malignant agents or inheritance from parent to child. These causes are those that lead to the excessive growth and multiplication of cells causing the disease. There are several types of cancer that affect women, including ovarian, uterine, vulvar, vaginal, cervical and breast cancers.

One of the known techniques for the diagnosis of breast cancer is the ELISA test, a blood test based on the interaction between antibodies and antigens, to subsequently be detected by enzymatic and reactive reactions if there are antigens present in the blood. The microplate reader that helps to read the absorbance can tell if there are antigens in the sample.

Microplate Readers

A microplate reader is the one in charge, with the help of an instrument called microplates, of reading the results of different types of tests, can make measurements of absorbance, luminescence and fluorescence. The optical system of the microplate reader uses fibers that give light to the wells where the samples are contained. The light emitted by the reader passes through the sample and, using its detection system, its absorbance is determined, once the light is amplified in the sample.

On the other hand, the microplates are rectangular in shape, have a dimension of 12.5 cm x 8.5 cm approximately. They have 12 columns and 8 rows with 96 microwells or wells in the form of V, U or planes, designed to contain the microlites of samples used for clinical diagnostic testing and analytical research.

Using the microplate reader, it is possible to read tests that have been performed in advance, for example, the ELISA test, an immunoenzymatic assay applied in the field of immunology and serology, which allows the detection of antigens or antibodies of a foreign agent in the body. Cancer can be detected by this technique.

Kalstein brand microplate reader

At Kalstein we are characterized by being MANUFACTURERS of a great variety of laboratory equipment, which also have excellent PRICES within the market, and which adapt to your pocket. We have the best microplate readers, equipped with excellent capabilities for all those researchers in the area of science and medicine. In our catalog you can find both microplate readers and Elisa readers.

If you are interested in making the PURCHASE of one of these, you can click on our link and make the quote: HERE

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