The spectrophotometer is a piece of equipment used in the laboratory in order to determine the concentration of a substance in a solution, thus allowing quantitative analysis to be carried out. An adequate calibration of the spectrophotometer is a fundamental factor to obtain an accurate and reliable measurement. We must bear in mind that having the spectrophotometer calibrated at all times will help us obtain much more precise information about the measurement we make.
In addition, in some cases, the device itself may not allow a measurement to be made if it is not previously calibrated. We must also take into account that the spectrophotometer, being a photosensitive device, can become uncalibrated due to sudden changes in ambient temperature or extreme temperatures. The calibration process, according to the instructions for use, must be carried out at most every 14 days. Our recommendation is to do it daily, since the investment of time in relation to its benefits is minimal.