Laboratory Spectrophotometers

A spectrophotometer is a piece of equipment used in laboratories to determine the beam of electromagnetic radiation or light and thus identify, qualify and quantify its intensity. In the same way, it allows to determine its efficiency, sensitivity, resolution and spectral range. Which will depend on the variabilities of the design and the choice of the optical components it contains. The spectrophotometer basically serves to know what is the concentration of substances in a solution and thus analyze under the quantitative approach. It is an instrument used in the laboratory whose main objective is to diagnose.

When to calibrate my spectrophotometer?

The spectrophotometer is a piece of equipment used in the laboratory in order to determine the concentration of a substance in a solution, thus allowing quantitative analysis to be carried out. An adequate calibration of the spectrophotometer is a fundamental factor to obtain an accurate and reliable measurement. We must bear in mind that having the spectrophotometer calibrated at all times will help us obtain much more precise information about the measurement we make.

In addition, in some cases, the device itself may not allow a measurement to be made if it is not previously calibrated. We must also take into account that the spectrophotometer, being a photosensitive device, can become uncalibrated due to sudden changes in ambient temperature or extreme temperatures. The calibration process, according to the instructions for use, must be carried out at most every 14 days. Our recommendation is to do it daily, since the investment of time in relation to its benefits is minimal.

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What do we recommend?

At Kalstein we urge you to follow these good practices for using the spectrophotometer, with which you will improve its performance and extend its life:

Carry out the calibration of the spectrophotometer, each time the analysis of a group of samples 

Keep the lid of the sample holder closed during the measurement process, to ensure an adequate reading.

Avoid reusing disposable cuvettes, to carry out this type of procedure in the laboratory, to give better results

Use only quartz cuvettes for analysis, to perform analysis below 310 nm.

Avoid the use of plastic cuvettes, if organic solvents are used in laboratory procedures

Use high quality borosilicate glassware to prepare the standards. Avoid using sodium glassware

Carefully clean glass cuvettes after use. Discard those that show scratches on the polished surface.

Use high quality reagents whenever possible. Low quality reagents can cause contamination

Verify that the samples or standards have not degassed inside the cuvettes.

When trying to use new procedures, not all substances comply with Beer's law.

Types of Spectrophotometer:

Knowing all the types of spectrophotometer will make its use much easier. In addition, what each one of them is used for, without having problems when identifying it with other instruments. There are different types of spectrophotometers, so that you can make good use of this instrument, I will show you below what their types are:

Double beam spectrophotometer

The light travels through two paths to the compartments, and each has its own detector. One goes to the sample and the other to the reference. Dual optical path, dual beam optical system, dual detector, with imported high performance grating, lower stray light, higher stability, reliability, more accurate analysis. Equipped with 10 “touch screen, built-in 32G memory, supports Bluetooth connection network. Dynamics functions, DNA / protein testing, multi-wavelength testing, and data printin

Single beam spectrophotometer

The light is transported from the sample to the detector, so a reference is needed to perform the analysis of the samples in a more optimal way. The instrument uses a lead screw structure so that the instrument wavelength accuracy and wavelength resolution can be greatly improved. Users set wavelength automatically through arrow keys to avoid operation errors. With microprocessor controlled, all parameters of the instrument can be easily set by numerical keys.


What is your ideal Spectrophotometer for the laboratory?

There are countless models, so it is normal that you do not know which laboratory equipment to buy that suits your needs. At Kalstein, we evaluate them to find what you're looking for.

When to calibrate my spectropho- tometer?

The spectrophotometer is a piece of equipment used in the laboratory in order to determine the concentration of a substance in a solution, thus allowing quantitative analysis. An adequate calibration ...

How to keep my laboratory  equipement in good condition?

The spectrophotometer is one of the main diagnostic and research instruments developed by humans. It is used in the laboratory in order to determine the concentration of a substance in a solution, thus allowing the realization...

What is a laboratory  Spectropho- tometer?

A spectrophotometer is a piece of equipment used in laboratories to determine what the beam of electromagnetic radiation or light is and thus identify, qualify and quantify its intensity...

What are the uses of a spectrophotometer?

The spectrophotometer quantifies the concentration of a specific substance that is present in a sample by comparing the amount of light entering a sample compared to the amount of light leaving the sample at a specific wavelength. It can be used theoretically to measure any substance that absorbs light.

Kalstein spectrophotometer in our catalogue:

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